以下YAC-1價格是細(xì)胞來源和細(xì)胞培養(yǎng)的詳細(xì)介紹:
產(chǎn)品名稱:YAC-1價格 數(shù)量:大量 細(xì)胞類型:其他細(xì)胞類型 是否是腫瘤細(xì)胞:0 物種來源:小鼠 運(yùn)輸方式:凍存運(yùn)輸 品系:A/Sn 生長狀態(tài):懸浮生長 細(xì)胞形態(tài):淋巴樣 ATCC Number:TIB-160? 相關(guān)疾病:淋巴瘤 規(guī)格:0.5mg Designations: YAC-1 Depositors: ?JY Djeu Biosafety Level:2 Shipped: frozen Medium & Serum: See Propagation Growth Properties:suspension Organism: Mus musculus Morphology:lymphoblast Source: Disease: lymphoma Strain: A/Sn Cell Type: Moloney murine leukemia virus (Mo-MuLV) induced Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Applications:assay of NK cell activity ( [22345 ] [49299 ] The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity.) Comments:Tested and found negative for ectromelia virus (mousepox). Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 2 X 10(5) and 2 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 Cell culture tested DMSO:ATCC 4-X References: 22345: Kiessling R, et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed: 1234049 YAC-1價格26348: Cikes M, et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed: 4573851 32552: Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012 49299: Kiessling R, et al. "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed: 1086218 YAC-1價格培養(yǎng)方法: 收到細(xì)胞后,在倒置顯微鏡下觀察整個細(xì)胞生長情況,如果細(xì)胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內(nèi),嚴(yán)格無菌操作,打開細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。 培養(yǎng)實(shí)驗又要開始了,科研工作者又該忙碌了,大家可能都在尋找適合自己的細(xì)胞,不用急,我們幫您掌舵,讓您滿意! 傳代方法:細(xì)胞*匯合時,倒掉舊液,加入消化液(0.25%胰蛋白酶+0.03%EDTA)2ml消化,顯微鏡下觀察細(xì)胞*脫離瓶壁分離成單個細(xì)胞后棄掉胰酶,加培養(yǎng)基混勻分瓶,T25瓶中加培養(yǎng)基至6-8ml,T75瓶加培養(yǎng)基至20ml,37℃,5%CO2孵箱培養(yǎng)。
鬼臼毒素 518-28-5 HPLC≥98%;20mg 訂購|咨詢 防己諾林堿 33889-68-8 HPLC≥98%;20mg 訂購|咨詢 粉防己堿 518-34-3 HPLC≥98%;20mg 訂購|咨詢 輔酶Q10 303-98-0 HPLC≥98%;20mg 訂購|咨詢 冬凌草甲素 28957-04-2 HPLC≥98%;20mg 訂購|咨詢 丹參素鈉 67920-52-9 HPLC≥98%;20mg 訂購|咨詢 隱丹參酮 35825-57-1 HPLC≥98%;20mg 訂購|咨詢 二氫丹參酮I 87205-99-0 HPLC≥98%;20mg 訂購|咨詢 丹參酮I 568-73-0 HPLC≥98%;20mg 訂購|咨詢 丹參酮IIA-磺酸鈉 UV≥98%;20mg 訂購|咨詢 丹參酮IIA 568-72-9 HPLC≥98%;20mg 訂購|咨詢 丹酚酸B 115939-25-8 HPLC≥98%;20mg 訂購|咨詢 丹酚酸A 96574-01-5 HPLC≥98%;20mg 訂購|咨詢 丹皮酚 552-41-0 HPLC≥98%;20mg 訂購|咨詢 薄荷醇 2216-51-5 HPLC≥98%;20mg 訂購|咨詢 虎杖苷 27208-80-6 HPLC≥98%;20mg 訂購|咨詢 白藜蘆醇 501-36-0 HPLC≥98%;20mg 訂購|咨詢 阿魏酸 1135-24-6 HPLC≥98%;20mg 訂購|咨詢 二氫楊梅素 27200-12-0 HPLC≥98%;20mg 訂購|咨詢 楊梅素 529-44-2 HPLC≥98%;20mg 訂購|咨詢 蘆薈大黃素 481-72-1 HPLC≥98%;20mg 訂購|咨詢 大黃酚 481-74-3 HPLC≥98%;20mg 訂購|咨詢 大黃素甲醚 521-61-9 HPLC≥98%;20mg 訂購|咨詢 ACY3 英文名稱: 丙型肝炎病毒核結(jié)合蛋白-1抗體 0.2ml ARSK 英文名稱: 芳香基硫酸酯酶K抗體 0.2ml Amphiphysin 英文名稱: 神經(jīng)元突觸前膜蛋白抗體 0.2ml Ankyrin G 英文名稱: 錨定蛋白G抗體 0.2ml Actin 英文名稱: 肌動蛋白α/α-SMA/α Actin抗體 0.1ml ADAMTS1 英文名稱: 整合素樣金屬蛋白酶與凝血酶1型抗體 0.1ml ADAMTS7(NT) 英文名稱: 整合素樣金屬蛋白酶與凝血酶1型-7抗體 (N端抗體) 0.1ml ADAMTS7 英文名稱: 整合素樣金屬蛋白酶與凝血酶1型-7抗體 0.1ml beta Adducin 英文名稱: 內(nèi)收蛋白抗體 0.1ml ADFP 英文名稱: 脂肪組織分化相關(guān)蛋白抗體 0.1ml Adiponectin Receptor 1 英文名稱: 脂聯(lián)素受體1抗體 0.2ml ADM R 英文名稱: 腎上腺髓質(zhì)素受體抗體 0.1ml Adiponectin 英文名稱: 脂聯(lián)素抗體 0.1ml ADM 英文名稱: 腎上腺髓質(zhì)素抗體 0.1ml Adenosine A3 Receptor 英文名稱: 腺苷受體A3抗體 0.1ml ADRA2 英文名稱: ADRA2腎上腺素能受體2抗體 0.1ml ADRB1 英文名稱: 腎上腺素能受體β1抗體 0.1ml ADRB2 英文名稱: 腎上腺素能受體β2/β2-AR抗體 0.1ml AGEs 英文名稱: 晚期糖基化終末產(chǎn)物抗體 0.1ml YAC-1價格Aggrecan1 英文名稱: 軟骨蛋白聚糖抗體 0.1ml ALAS-E 英文名稱: 5-氨基乙酰丙酸合酶1抗體 0.2ml ASPP1 英文名稱: p53凋亡刺激蛋白1抗體 0.1ml ASPP2 英文名稱: P53凋亡刺激蛋白2抗體 0.1ml ARIP2B 英文名稱: 激活素受體2B抗體 0.2ml YAC-1價格在運(yùn)輸?shù)倪^程中,細(xì)胞培養(yǎng)瓶中的貼壁細(xì)胞有可能從瓶壁中脫落下來,因此客戶在收到貨以后的*時間是要先觀察產(chǎn)品的狀況,顯微鏡下觀察會出現(xiàn)細(xì)胞懸浮的情況,出現(xiàn)此狀態(tài)時,請不要立即打開培養(yǎng)瓶,應(yīng)立即將培養(yǎng)瓶置于細(xì)胞培養(yǎng)箱過夜,并于次日在顯微鏡下觀察,此時多數(shù)細(xì)胞會重新貼附于瓶壁。如果發(fā)現(xiàn)細(xì)胞仍不能貼壁,請試著用臺盼藍(lán)染色法鑒定細(xì)胞活力,如臺盼藍(lán)染色證實(shí)細(xì)胞活力正常請按懸浮細(xì)胞的方法處理;如若證實(shí)細(xì)胞無活力,請在收到貨后48小時內(nèi)將細(xì)胞狀態(tài)反饋給我公司,我們將盡快幫助解決。 |